Core Facility (Houghton'04)


-2008.."..just the FACS Ma'am!".	7500 Fast systems. The Applied Biosystems 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes. Fast optical plates ensure excellent precision in 10-30 uL reaction volumes which means savings on expensive reagents. Deconvolution upgrade. This upgrade using a new UNIX based OS, allows the user to image material in 4D at high resolution (6.7 mm pixels). A CoolSnap HQ2 CCD camera allows the software to apply sophisticated algorithms to remove out of focus light and reassign refracted light to the calculated point of origin. Beckman Biomek NX Laboratory Automation system. Biomek NX puts every aspect of liquid handling – including pipetting, dilution, dispensing, and integrations – into a single, automated system that is powerful and flexible. The NXP workstation offers multiple configurations, including multichannel, BBiomek NX features • Span-8 with gripper. The Span 8 for rapid plate-to-plate; tube to plate applications and assay miniaturization. • Rotate a full 360°. • Pipetting performance for low-volume reaction setup and assay miniaturization, as well as accurate and repeatable results extending into the submicroliter range. l FujiFilm FLA7000 Bioimaging analyzer. coming summer 2008 FLA-7000 is an advanced multipurpose bio-imaging analyzer which enables radioisotopic, fluorescent and digitized image capturing. With maximum 4 kinds of onboard fluorescence excitation lasers, this system is able to cover a broader range of fluorescent dyes including near-infrared. • Radioactive samples: 14C, 32P, 33P, 35S, 3H, Neutron • 4 available lasers: 473nm, 532nm, 635nm and 671nm • Fluorescent dyes: 473nm: Cy™2, FITC, SYPRO® Ruby, SYPRO® Orange, ECL plus™, silver stain, SYBR® Green I, SYBR® Green II, SYBR® safe, SYBR® Gold, FAM™, Alexa Fluor®488, ECF™ 532 nm: Cy™3, EtBr, ROX™, HEX™, TAMRA™, Alexa Fluor®546, CBB 635nm: Cy™5, Alexa Fluor®633 670nm: Alexa Fluor®680
BD Biosciences FACSAria Cell Sorter. The BD FACSAria™ cell sorter sets a new standard for high performance flow cytometry. This benchtop system delivers high-speed sorting and multicolor analysis. The system has three air-cooled lasers at 488-nm, 633-nm, and 405-nm, wavelengths. Two- and four-way bulk sorting devices for a variety of tube sizes are also available. Beckman Cell Lab Quanta SC . Cell Lab Quanta SC is a flow cytometry system that simultaneously measures electronic volume, side scatter and three fluorescent colors to provide population resolution and accurate cell counting. This flow cytometer has multiple excitation wavelengths, including 488 nm laser and a UV light source optimized for excitation at 366, 405, 435 nm. Side scatter and Coulter volume make size analysis and fluorescent measurements more precise. Perkin Elmer Victor3 plate reader. VICTOR X3 includes Luminescence, Fluorescence Intensity (both top and bottom reading) and UV-absorbance (UV-VIS) technologies. Technologies and reading modes *Fluorescence Intensity (340-850 nm)** • Fluorescence Polarization (400-850* nm) • Time-Resolved Fluorescence (TRF) • Luminescence • Glow, flash and dual luminescence • Absorbance (340-800 nm) • UV Absorbance (260 nm/280 nm) VICTOR3 comes with several standard features such as temperature control, shaking, area scanning, adjustable measuring height, below fluorescence reading, dual label/ratio reading and kinetics. A dispenser is also available for cell-based assays by enabling fast kinetics measurements, enzyme assays and numerous other cellbased drug discovery assays. Ultra-Lum Omega Molecular Imaging System. coming summer 2008 The Omega Molecular Imaging and Analysis System is a bench top imaging instrument. Equipped with a high resolution cooled scientific CCD camera, multi-wavelength LED epi-illumination,variable intensity UV transilluminator and a 6-position, motorized filter wheel.
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This information is given as a guide to the facilities and instrumentation available in the DNA/Protein Core facility at Georgia State University. If you have any concerns or thoughts about the content of this website please contact: John Houghton (404) 413-5390