A List of the Major Items of Equipment within the Core
Select an application topic and the appropriate equipment will open. Whenever there is a 
next to the name of a given
piece of equipment, please click
on the thumbnail to view an image of the equipment as well as a summary
of it's potential uses.
| Biology Applications | ||
| Basic | Equipment covered in Training Class |
|
| Support | ||
| DNA/RNA | ||
| Protein/Proteomics | ||
| DNA/RNA/Protein | ||
454 Sequencing - GS Junior, Roche |
| GS Junior sequencer uses 454 sequencing technology to perform high throughput sequencing. |
Analytical Ultracentrifuge,Optima
XL-A, Beckman |
|
subjects a sample to a centrifugal field while detecting and recording the resulting movement of molecules.This system has proven to be extremely accurate in the prediction of protein subunit and protein/DNA interactions. |
Atomic
Force Microscope, Park Scientific |
|
is able to delineate the topological characteristics, charge distribution and “texture” of macromolecular structures such as proteins and nucleic acids, with up to 2 -5 Angstrom resolution. |
AFM/Scanning Probe Microscope, Veeco |
|
is able to delineate the topological characteristics, charge distribution and “texture” of macromolecular structures such as proteins and nucleic acids, with up to 2 -5 Angstrom resolution. |
Avanti J-20 centrifuge, Beckman |
|
separates solutions on the basis of the particle size and density difference between the liquid and solid phases. |
|
BIAcore 2000 |
measurement of molecular interaction energetics and kinetics without the requirement of any sample labeling. This technology allows users to measure rapidly the relative affinity, specificity, allostery, and kinetics of biomolecular complexation | |
|
BIAcore 3000 |
|
measurement of molecular interaction energetics and kinetics without the requirement of any sample labeling. This technology allows users to measure rapidly the relative affinity, specificity, allostery, and kinetics of biomolecular complexation |
Bioanalyzer 2100, Agilent |
| “lab-on-a-chip” systems, for the quantitative analysis of DNA, RNA and proteins, that surpass gel-based electrophoretic systems for speed and ease of use. |
Biomek
2000 Automation Workstation, Beckman
|
|
provides simple, intelligent automation of liquid-handling tasks, such as Pipetting, diluting and dispensing operations |
Biomek
NXp Automation Workstation, Beckman
|
|
Biomek NX puts every aspect of liquid handling – including pipetting, dilution, dispensing, and integrations – into a single, automated system. Biomek NX features Span-8. The Span 8 is used for rapid plate-to-plate; tube to plate applications and assay miniaturization. |
CHEF
DR II pulse wave, BioRad |
|
effectively resolves DNA fragments in the range of 5 kb to 6 Mb and is used for disease locus mapping, YAC, BAC, PAC, and cosmid mapping, RFLP and DNA fingerprinting and Chromosome rearrangements. |
Ettan
DALT II system; IPGphor
|
|
enables isoelectrically focused separation of proteins on appropriately prepared IEF strips . |
Ettan
DALT II system; Separation Unit |
|
performs second-dimension SDS-PAGE separations and separates proteins by molecular weight |
Ettan
DALT II system; Typhoon 9400
|
|
creates a scanned image of 2D gels, that have been pre-stained Cy-dye fluorescent chemistry or post-stained with Deep Purple fluorescent chemistry |
Ettan
DALT II system; Spot Picker
|
|
provides for the automated excision of proteins from the 2D gel, and their transfer to a microplate. |
Ettan
DALT II system; Digester
|
|
In-gel digestion of proteins from 2-D electrophoresis gel plugs from the Ettan Spot Picker microplate. |
Gamma Counter , Wallac 1470 |
|
designed for all laboratory gamma work—with an energy range up to 900 keV. Isotopes that can be counted are: 58Co, 59Fe, 57Co, 51Cr, 22Na, 111In, 137Cs and 134C |
Gene
Pulse and Micropulser Electroporation System, BioRad |
|
provides a highly efficient method for introducing nucleic acids, proteins and other molecules into cells. |
Genetic Analyzer 3730, ABI
|
|
automated capillary electrophoresis system that can separate, detect and analyze up to 48 capillaries of fluorescently labeled DNA fragments in 1 run. |
Gilson 215 Liquid Handler |
| a single-probe, "workhorse" liquid handler that delivers a robust performance for fast and accurate direct injection onto HPLC or LC/MS systems |
MALDI TOF/TOF Analyzer 4800 Plus, ABI |
|
one of the most sensitive protein / biomarker discovery systems currently available using time-of-flight MS/MS technology. |
MacConnell automated plasmid DNA purification system |
|
Without centrifugation, the system can run up to 48 mini-preps per hour and the run is truly walk away. |
Phosphoimager, FLA
7000 GE Healthcare |
|
an advanced multipurpose bio-imaging analyzer which enables radioisotopic, fluorescent and digitized image capturing. |
|
Omega 10g Imaging Software, Ultra -Lum |
|
can analyze DNA, RNA and protein, single bands, multi-color fluorescence 1-D and dot blots. Result analysis can include standard measurements of mass, molecular weight, relative abundance and measurements can be standardized |
Plate Reader, Enspire, Perkin-Elmer |
|
reads optical biosensor label-free, fluorescence intensity, absorbance, ultra-sensitive luminescence and Alpha technologies in microplates up to 384-wells. | Plate Reader, SPECTRAmax Plus 384 , Molecular Devices |
|
The SpectraMax® Plus384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes. |
Plate Reader, SPECTRAMax /BioMek |
|
filter-based microplate spectrophotometer provides rapid and sensitive measurements of a variety of analytes across a wide range of concentrations in. |
Plate Reader, Victor 3, Perkin-Elmer |
|
includes Luminescence, Fluorescence (both top and bottom reading) and UV-absorbance (UV-VIS) technologies. |
Prism
7500 Fast Real Time PCR System, ABI |
|
monitors the progress of the PCR as it occurs using probes labeled with various fluorophores.The Fast systems reactions are only 10 ul and can be run in 30 - 40 min. |
Simple Simon Western Assay, Protein Simple |
|
Gel-free, Blot-free, Hands-free automated Western Blots. |
Typhoon 9400/Typhoon Trio, GE Healthcare |
|
creates a scanned image of 2D gels, that have been pre-stained Cy-dye fluorescent chemistry or post-stained with Deep Purple fluorescent chemistry |
UV Crosslinker |
|
The UV crosslinker covently binds nucleic acids to membranes in 30 sec. or less. |
Vacufuge Concentrator 5301, Eppendorf |
|
concentrates DNA/RNA, nucleotides, proteins, and other samples quickly; has a true RT mode |
| Cell | ||
| Chromatography | ||
| Genomics | Software | |
| Imaging | ||
| Chemiluminescent Imaging | ||
| Fluorescent Imaging | ||
| Gel Documentation | ||
| Molecular Imaging | ||
| Image Quantitation/Analysis | ||
| Other Core Facilities Applications | ||
| Fermentation | Fermentation and Cell Breakage | |
| Kinetic Analysis | ||
| Biological Microcalorimetry | ||
| Surface Plasmon Resonance | ||
| Microscopy and Imaging | Microscopy | |
| Histology | ||
| Imaging | ||
| Neuroscience Institute | Histology | |
| Imaging | ||
| Modeling | ||
| Molecular Modeling | ||
| Spectrometric Analysis | ||
| Mass Spectrometry | ||
| NMR Spectrometry | ||
| Spectroscopy | ||
This information is given as a guide to the facilities and instrumentation available in the DNA/Protein Core facility at Georgia State University. If you have any concerns or thoughts about the content of this website please contact: John Houghton (404) 413-5390
