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Fuji Phosphoimager MacBAS 2500 |
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| Contact: Debby Walthall Rm: NSC 338 Tel: (404) 413-5363 |
General Warnings: Every time you use the phosphoimager, you MUST sign the logbook. Do not leave the Imaging Plate (IP) in the reader any longer than necessary. Once you have erased your IP, take it back to your lab. Do not store any images on the hard drive. If you need to, save your image to a disk. Do not under any circumstance, pull the IP out of the scanner drawer – let it come out on it’s own (see below **) |
| 1. IP reader is usually on with only the power light on (green). 2. Turn IP reader on, switch in back on the right hand side. 3. Wait for start up adjustment/self diagnosis to finish - ~5 – 15 minutes. The center scan light comes on during this startup and will go out when the reader is reader to use. 4. Snap open reader lid. Place IP with white side up into reader. Close lid. 5. Image Reader should be up on computer desktop. If not open it with the launcher Image Reader icon in the launcher at the bottom. 6. Image Reader window. Make sure that Read and Launch Image is selected (pull down list at bottom middle of window). 7. Read. Another window comes up so that you can select where you want your image to be saved. READ. 8. You can see image as it’s scanned. OK. Red scan light comes on. 9. ** Plate slides into scanner. When scan is done it will automatically slide out (to location you put the plate). Your plate is back to it’s original spot when the red scan light turns off. The scan is complete and then it takes a minute or so for the light to go out. DO NOT open the door and pull your plate out of the scanner slot. 10. Image comes up in Image Gauge program. You can also go to File/Open/find saved image/open 11. Resize image. There’s a + sign in the image. Move the + to the right/left edge of your image and move it in or out, up or down on the up/down edge. You want your image size to be as small as you can get it and still have your data. This will save disk space 12. You can also flip/rotate image, by using the appropriate button. OK 13. Image/brightness contrast/exponential. A window appears that is called change curve. Put cursor on straight vertical line on right hand side of graph. Move the line right or left to get the brightness or contrast you want. OK 14. You can print you image or save it to a disk. If you print, you might want to bring paper to use in the printer – it quite often is out. 15. After you are done, place the IP in the plate eraser. Usually 5 minutes is enough time to erase the plate. If the the plate is overexposed, you might need up to 40 min. If you are worried that the plate might not be clean, rescan the plate. 16. The IP should be stored in it’s plastic folder so that the edges don’t curl. If you have to, wipe it off with a KimWipe or if it’s really dirty, wipe with EtOH. Expose IP in the dark. Do not use any H2O or detergent on the plate. This information is given as a guide to the facilities and instrumentation available in the DNA/Protein Core facility at Georgia State University. If you have any concerns or thoughts about the content of this website please contact: John Houghton (404) 413-5390
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