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Beckman Avanti J-20 XP1
1. Turn power on.
2. Open door with foot latch near floor on right.
3. Put rotor in and tighten lid
4. Rotor selection should automatically change when you put a new rotor in.
5. Punch speed button. Select either rpm or rcf with buttons on right. Type in speed and then enter.
6. Punch time button. Select either time in HH:MM, W2T (whatever that is) or hold with buttons on right. Type in time if time button selected and then enter.
7. Punch Temp. button. Type in temp. and then enter.
8. Start
9. Stop if hold button has been selected.
10. Use foot latch to open door.
11. Turn centrifuge off.
Eppendorf Centrifuge 5804R 1. Turn centrifuge on, switch on right side near bottom front.
2. Use fast cool to cool chamber and rotor quickly. Make sure there isn’t a blank in rotor before starting fast cool because the rotor spins.
3. Punch speed. Speed will start flashing. Use up and down arrows to adjust speed. Punch speed again to switch between rpm, rcf and g.
4. Punch time. Time will start flashing. Use up and down arrows to adjust Time.
5. Punch Temp. Temp. will start flashing. Use up and down arrows to adjust Temp.
6. Punch Open to open door of chamber.
7. After samples in, close door and punch start.
8. When done, punch door to open centrifuge door.
9. You can change out the rotor. The microfuge tube rotor is on the table to the left of the centrifuge. To loosen the screw holding the rotor in place, use the tool that is in the top drawer to the left of the centrifuge. The tool is long and has a black handle labeled AMF.
10. The screw to loosen is in the middle of the rotor. Take the rotor out and place on the table. Put the other rotor in and tighten the screw. Be sure to put the tool back in the drawer. The centrifuge doesn’t immediately recognize a rotor change. Start the centrifuge and then stop it. This start/stop triggers the rotor recognition. Now you can change speed for the new rotor.
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This information is given as a guide to the facilities and instrumentation available in the DNA/Protein Core facility at Georgia State University. If you have any concerns or thoughts about the content of this website please contact: John Houghton (404) 413-5390
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