Proteomics General Supplies 1st Dimension 2nd Dimension Visualization Please print out and fill in the form below, and return to Hyuk-Kyu Seoh. |
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Following prices apply to academic institutions. Contact Core Personnel for other pricing. |
Charges : |
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Gel Services: |
$80.00/gel (includes 2D gel set-up, supplies, scan, analysis and spot picking) |
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does not include cost of staining ($10.00 - $180.00 per gel) |
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Gel Services: Large gel format |
$120.00/gel (includes 2D gel set-up, supplies, scan, analysis and spot picking) |
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does not include cost of staining ($10.00 - $180.00 per gel) |
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MALDI MS: |
Less than 5 samples | |
$50 per spot - includes gel pick, in-gel digest, ZipTip cleanup, spotting, MS and MS/MS analysis | ||
MALDI MS: |
More than 5 samples |
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$100.00 setup and $30.00 per spot - includes gel pick, in-gel digest, ZipTip cleanup, spotting, MS and MS/MS analysis | ||
MS analysis: |
$14.00 per spot - MS and MS/MS analysis
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MS analysis: |
$50.00 per analysis - in depth MS profile comparison and analysis
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| Proteomics Request Form | |||
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Biotechnology & Drug Design Core Facility, Georgia State University |
Contact: Hyuk-kyu Seoh Petit Science Center, 161 Jesse Hill Jr. Dr., Atlanta, GA 30303 |
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Contact Name: |
Contact Phone: |
E-mail: |
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Department/University: |
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Address: |
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P I's Name: |
Acct / PO # | ||
P I's Signature: |
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Sample Name: |
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Estimated amount (ug) ______________________________ |
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Protein Purity ________________________________ |
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Form (solution, gel, gel type, stain and method) ________________ |
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______________________________ |
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Estimated MW (if known) ______________________________ |
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Is sample a Biohazard (Y/N) ____________________________ |
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If Yes, please explain: _________________________________ |
Sample is dissolved in: ________________________________ |
Any additional sample Information: ____________________ |
_______________________________ |
Sample Preparation: |
Sample source (tissue, cell culture, etc.) __________________ |
Homogenization buffer used: _______________________ |
(SDS and Triton-X not recommended) |
Cell Disruption method used: _______________________ |
Protein ppt. (Y/N) and method if yes: ___________________ |
Protein quantitation used: ____________________________ |
Sample is soluble in (MeOH, H2), CH3CN): ______________ |
Sample degrades in: __________________________________ |
1st Dimension: |
pI (if known) _________________________________ |
Reduced (Y/N) __________ Alkylated (Y/N) __________ |
pH range preferred: _________________________________ |
CyDyes (Y/N) and if Yes which ones: ___________________ |
If No, what method of detection: _______________________ |
Rehydration solution used: ___________________________ |
2nd Dimension: |
Equilibrium solution used: ___________________________ |
%SDS-PAGE gel: ________________________________ |
~Mass range: __________________________________ |
Visualization |
Staining method: ______________________________ |
Scan: _________________________________ |
Spot Picker used (Y/N): ______________________ |
If yes, reference marker must be on plate |
Scan Analysis: _____________________________ |
Spot Picker |
Pick List file name and location: ___________________ |
Protein ppt. (Y/N) and method if yes: ___________________ |
Trypsin digest |
Digestion method preferred: ___________________________ |
Zip Tip (Y/N): ___________________________ |
MALDI |
Biological Source: ___________________________ |
Database search parameters: _________________________ |
Is the Protein in Database (Y/N): _____________________ |
If Yes, give db and id # (ex. emb CAA65167.1): _________ |
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Special Instructions: _______________________________ |
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| Supplies General Supplies 1st Dimension 2nd Dimension Visualization Supply cost is to be used only as a guide. It is a website price (as of 1/3/07) and might not include any University discounts, shipping and handling. |
Item
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Description |
Company |
Catalog # |
Unit |
Cost/Unit |
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General Supplies |
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Crew Wipes |
11.5 in. X 12 in.; very absorbent, soft so won’t scratch surfaces |
Fisher |
06-666-12 |
50/PK |
$43.48 |
Crew Wipes |
9 in. X 9 in.; very absorbent, soft so won’t scratch surfaces
|
Fisher |
06-666-26 |
50/pk |
$17.37 |
Contrad 70 (detergent) |
general purpose detergent; cleans any surface, leaves no detectable residues; phosphate-free, chlorine-free |
Fisher |
04-355-1 |
5 L |
$91.42 |
| Triton X-100 | detergent that can be used instead of CHAPS |
GE Healthcare |
17-1315-01 |
500 ml |
$40.00 |
| 2-D Quant Kit | Accurately determine protein concentration |
GE Healthcare |
80-6483-56 |
500 assays |
$219.00 |
2-D Clean-Up Kit
|
Improves quality of 2D results by removing interfering contaminants |
GE Healthcare |
80-6484-51 |
50 samples |
$219.00 |
| BCA Protein Assay Kit | detergent compatible assay for the colorimetric detection and quantitation of total protein |
Pierce |
23227 |
250 assay |
$87.00 |
Urea
|
protein solubilization; used in rehydration soln., cell lysis , 2X sample buffer and equilibrium buffer |
GE Healthcare |
17-1314-01 |
500 gm |
$33.00 |
CHAPS
|
protein solubilization; used in rehydration soln., cell lysis buffer and 2X sample buffer. |
GE Healthcare |
17-1314-01 |
1 GM |
$59.00 |
Thiourea
|
solubilizes and denatures proteins, using thiourea in addition to urea can further improve solubilization particularly membrane proteins; used in rehydration soln. and 2X sample buffer. |
GE Healthcare |
RPN6301 |
100 gm |
$52.00 |
| Iodoacetamide | alkylation of thiols preventing reoxidation, which can result in streaking and other artifacts; minimize unwanted reactions of cysteine residues if MALDI will be done later; alkylates residual DTT to revent point streaking and other silver-staining artifacts; used in 2nd equilibrium buffer |
GE Healthcare |
RPN6302 |
25 gm |
$83.00 |
Dithiothreitol (DTT)
|
reductant that cleaves disulphide bonds to allow proteins to unfold completely; used in 2X gel loading buffer, rehydration buffer and 2X sample buffer |
GE Healthcare |
17-1318-01 |
1 gm |
$33.00 |
DNPH |
a chemical compound that can be used in a chemical test to detect whether an organic compound has a C=O (carbonyl) group. |
Sigma |
D-199303-25G |
25 gm |
$21.80 |
| Bromophenol Blue |
Tracking dye for 1st and 2nd dimension |
GE Healthcare |
17-1329-01 |
10 gm |
$35.00 |
Cy Dye DIGE Fluor |
CyDye™ DIGE fluors are dyes for multicolor analysis, offering bright and intense colors with narrow excitation and emission bands. The fluors are spectrally distinct, making them ideal for multicolor detection. They are also available in 25 nM and 5 nM quantities |
GE Healthcare |
25-8008-60 |
10 nM/vial |
$1526.00 |
Cy Dye DIGE Fluor |
GE Healthcare |
25-8008-61 |
10 nM/vial |
$1526.00 |
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Cy Dye DIGE Fluor |
GE Healthcare |
25-8008-62 |
10 nM/vial |
$1526.00 |
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Immobiline™ DryStrip gels
13 cm,pH 3-5.6 NL |
there are 24cm, 18cm, 13cm and 7 cm also available in the same pH ranges. |
GE Healthcare |
17-6003-55 |
12 strips |
$108.00 |
Immobiline™
DryStrip 13 cm, pH 5.3-6.5 NL |
GE Healthcare |
17-6003-60 |
12 strips |
$108.00 |
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Immobiline™ DryStrip 13 cm,
pH 6.2-7.5 NL |
GE Healthcare |
17-6003-65 |
12 strips |
$108.00 |
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Immobiline™ DryStrip 13 cm,
pH 7-11 NL |
GE Healthcare |
17-6003-70 |
12 strips |
$108.00 |
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Immobiline™ DryStrip 13 cm,
pH 3-11 NL |
GE Healthcare |
17-6003-75 |
12 strips |
$108.00 |
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Immobiline™ DryStrip 13 cm,
pH 4-7 NL |
GE Healthcare |
17-6001-13 |
12 strips |
$108.00 |
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Immobiline™ DryStrip 13 cm,
pH 6-11 NL |
GE Healthcare |
17-6001-96 |
12 strips |
$108.00 |
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Immobiline™ DryStrip 13 cm,
pH 3-10 NL |
GE Healthcare |
17-6001-15 |
12 strips |
$108.00 |
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Immobiline™ DryStrip 13 cm,
pH 3-10 |
GE Healthcare |
17-6001-14 |
12 strips |
$108.00 |
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IPG Buffer, pH 4-7 |
For use with Immobiline™ DryStrip gels to improve protein solubility. The buffer is specific for whatever pH range you buy. If you use Cy Dyes, you have to use Pharmalyte (see below). Ensures optimal conditions for IEF separation. Eliminates background staining. |
GE Healthcare |
17-6000-86 |
1 ml |
$47.00 |
IPG Buffer,
pH 3-10 |
GE Healthcare |
17-6000-87 |
1 ml |
$47.00 |
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IPG Buffer,
pH 3-11 NL |
GE Healthcare |
17-6004-40 |
1 ml |
$51.00 |
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IPG Buffer, pH 6-11 |
GE Healthcare |
17-6001-78 |
1 ml |
$47.00 |
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IPG Buffer,
pH 7-11 NL |
GE Healthcare |
17-6004-39 |
1 ml |
$51.00 |
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Pharmalyte, broad range
pH 2.5-5 |
For use with Immobiline™ DryStrip gels to improve protein solubility. The buffer is specific for whatever pH range you buy. If you use Cy Dyes, you have to use Pharmalyte. Ensures optimal conditions for IEF separation. Eliminates background staining. |
GE Healthcare |
17-0451-01 |
25 ml |
$244.00 |
Pharmalyte, broad range
pH 4-6.5 |
GE Healthcare |
17-0452-01 |
25 ml |
$244.00 |
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Pharmalyte, broad range pH 5-8
|
GE Healthcare |
17-0453-01 |
25 ml |
$244.00 |
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Pharmalyte, broad range
pH 8-10.5 |
GE Healthcare |
17-0455-01 |
25 ml |
$244.00 |
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Pharmalyte, broad range
pH 3-10 |
GE Healthcare |
17-0456-01 |
25 ml |
$244.00 |
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Pharmalyte, Narrow range
pH 4.2-4.9 |
GE Healthcare |
17-0562-01 |
25 ml |
$297.00 |
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Pharmalyte, Narrow range
pH 4.5-5.4 |
GE Healthcare |
17-0563-01 |
25 ml |
$297.00 |
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Pharmalyte, Narrow range
pH 5-6 |
GE Healthcare |
17-0564-01 |
25 ml |
$297.00 |
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Pharmalyte, Narrow range
pH 6.7-7.7 |
GE Healthcare |
17-0566-01 |
25 ml |
$297.00 |
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IPGphore Strip Holder Cleaning Soln. (nonionic)
|
nonionic detergent for cleaning strip holders and covers |
GE Healthcare |
80-6452-78 |
950 ml |
$48.00 |
Immobiline DryStrip Cover Fluid
|
oil used to cover Immobiline DryStrip and sample for the 1st dimension to prevent drying out strip |
GE Healthcare |
17-1335-01 |
1 L |
$53.00 |
DeStreak Rehydration Solution | Improves reproducibility and quallity fo 2-D gels by preventing streaking |
GE Healthcare |
17-6003-19 |
5 X 3 ml |
$89.00 |
Bind Silane
|
Binds the gel to 1 plate. You need all of the gel on a specific plate for spot picking. |
GE Healthcare |
17-1330-01 |
25 ml |
$92.00 |
Protogel
|
for 2nd dimension vertical gels |
National Diagnostics |
EC-890 |
1 L |
$67.93 |
Ammonium persulfate
|
Add to the Protogel for gel polymerization |
GE Healthcare |
17-1311-01 |
25 gm |
$35.00 |
TEMED
|
Add to the Protogel for gel polymerization |
GE Healthcare |
17-1312-01 |
25 ml |
$29.00 |
Plastic trays for gels/glass plates
|
multiple uses; storing 2nd dimension gels and staining 2D gels |
VWR |
62650-129 |
6/cs |
$49.36 |
Gel Releasers
|
Helps pry the 2nd dimension gel sandwich apart |
Bio-Rad |
165-3320 |
5/PK |
$22.00 |
SDS
|
use in equilibration solution and running buffer for 2nd dimension; protein solubilization |
Sigma |
17-1313-01 |
100 gm/ea. |
$40.00 |
Glycerol
|
Stabilizes the pH gradient and stabilizes 2nd D gels |
GE Healthcare |
17-1325-01 |
1 L |
$49.00 |
Glycine
|
use in running buffer for 2nd dimension |
GE Healthcare |
17-1323-01 |
500 gm |
$35.00 |
Tris
|
GE Healthcare |
17-1321-01 |
500 gm |
$60.00 |
|
ProtoMarkers
|
Protein marker |
National Diagnostics |
EC-898 |
500 ul |
$81.00 |
Deep Purple Protein Stain
|
Deep Purple™ Total Protein Stain* is an ultra-sensitive, fast, and easy-to-use fluorescent stain for the detection of proteins in gels or blots following electrophoretic separation. It is similar to sypro ruby but gives nicer spots and is a bit cheaper than sypro, though not quite as sensitive. Very similar to SYPRO Ruby |
GE Healthcare |
RPN6305 |
5 ml (makes 1 L) |
$168.00 |
Citric Acid
|
new Deep Purple protocol |
Sigma |
C0759-500G |
500 gm |
$22.50 |
| Boric Acid | new Deep Purple protocol |
Fisher |
A73-500 |
500 gm |
$75.05 |
SYPRO Ruby
|
very sensitive fluorescent stain (8-28 ng range) for protein gels that can be detected by UV fluorescence. Very similar to Deep Purple Protein Stain |
Molecular Probes (Invitrogen) |
S12001 |
200 ml |
$67.00 |
Krypton Protein Stain
|
Fluorescent stain, almost as sensitive as Deep Purple and SYPRO Ruby; better background |
Fisher |
P153071 |
100 ml |
$82.70 |
ProteoSilver Silver Stain kit
|
Stable and sensitive silver staining kit |
Sigma |
PROTSIL1-KT |
25 mini gels |
$135.50 |
Coomasie Brilliant Blue R-250
|
commonly used stain for protein gels; inexpensive and rapid but low sensitivity (100- 1000ng range) |
Bio-Rad |
161-0400 |
10 gm |
$18.00 |
SimplyBlue SafeStain
|
Fast, sensitive and safe Coomassie G250 stain |
Invitrogen |
LC6060 |
1 L |
$100.00 |
GelCode Blue Stain Reagent
|
Coomassie G250 stain without multiple steps for destaining |
Pierce |
24590 |
500 ml |
$55.00 |
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