Proteomics
2D Electrophoresis guides

Supply list
General Supplies
1st Dimension
2nd Dimension
Visualization

Please print out and fill in the form below, and return to Hyuk-Kyu Seoh.

Rm (lab): PSC 537

Rm (office): PSC 521
Tel: (404) 413-5379

 
 
Following prices apply to academic institutions.
Contact Core Personnel for other pricing.
Charges : 
 

Gel Services:
Small gel format 

$80.00/gel (includes 2D gel set-up, supplies, scan, analysis and spot picking)
 
does not include cost of staining ($10.00 - $180.00 per gel)
Gel Services:
Large gel format 
$120.00/gel (includes 2D gel set-up, supplies, scan, analysis and spot picking)
 
does not include cost of staining ($10.00 - $180.00 per gel)
MALDI MS: 
Less than 5 samples
 
$50 per spot - includes gel pick, in-gel digest, ZipTip cleanup, spotting, MS and MS/MS analysis
MALDI MS: 
More than 5 samples
 
$100.00 setup and $30.00 per spot - includes gel pick, in-gel digest, ZipTip cleanup, spotting, MS and MS/MS analysis
 
MS analysis: 
$14.00 per spot - MS and MS/MS analysis
MS analysis: 
$50.00 per analysis - in depth MS profile comparison and analysis
 
Proteomics Request Form
 
Biotechnology & Drug Design Core Facility, Georgia State University
 
Contact: Hyuk-kyu Seoh
Petit Science Center, 161 Jesse Hill Jr. Dr., Atlanta, GA 30303
Contact Name:
 
Contact Phone:
 
E-mail:
 
Department/University:
 
Address:
 
 
P I's Name:
  Acct / PO #  
P I's Signature:
 
 
Sample Name:
 
Estimated amount (ug)   ______________________________
Protein Purity   ________________________________
Form (solution, gel, gel type, stain and method)   ________________
______________________________
Estimated MW (if known)  ______________________________ 
Is sample a Biohazard (Y/N) ____________________________
If Yes, please explain:  _________________________________
Sample is dissolved in:   ________________________________
Any additional sample Information:   ____________________
_______________________________
Sample Preparation:
Sample source (tissue, cell culture, etc.)  __________________
Homogenization buffer used:  _______________________
(SDS and Triton-X not recommended)
Cell Disruption method used:  _______________________
Protein ppt. (Y/N) and method if yes:  ___________________
Protein quantitation used:  ____________________________
Sample is soluble in (MeOH, H2), CH3CN):  ______________
Sample degrades in:  __________________________________
1st Dimension:
pI (if known)  _________________________________
Reduced (Y/N) __________  Alkylated (Y/N) __________
pH range preferred:  _________________________________
CyDyes (Y/N) and if Yes which ones:  ___________________
If No, what method of detection:  _______________________
Rehydration solution used:  ___________________________
2nd Dimension:
Equilibrium solution used:  ___________________________
%SDS-PAGE gel:  ________________________________
~Mass range:  __________________________________
Visualization
Staining method:  ______________________________
Scan:  _________________________________
Spot Picker used (Y/N):  ______________________
If yes, reference marker must be on plate
Scan Analysis:  _____________________________
Spot Picker
Pick List file name and location:  ___________________
Protein ppt. (Y/N) and method if yes:  ___________________
Trypsin digest
Digestion method preferred: ___________________________
Zip Tip (Y/N): ___________________________
MALDI
Biological Source: ___________________________
Database search parameters: _________________________
Is the Protein in Database (Y/N):  _____________________
If Yes, give db and id # (ex. emb CAA65167.1): _________
_______________________________
Special Instructions: _______________________________
_______________________________
 
Supplies
General Supplies
1st Dimension
2nd Dimension
Visualization


Supply cost is to be used only as a guide. It is a website price (as of 1/3/07) and might not include any University discounts, shipping and handling.
Item
Description
Company
Catalog #
Unit
Cost/Unit
General Supplies
Crew Wipes
11.5 in. X 12 in.; very absorbent, soft so won’t scratch surfaces
Fisher
06-666-12
50/PK
$43.48
Crew Wipes
9 in. X 9 in.; very absorbent, soft so won’t scratch surfaces
Fisher
06-666-26
50/pk
$17.37
Contrad 70 (detergent)
general purpose detergent; cleans any surface, leaves no detectable residues; phosphate-free, chlorine-free
Fisher
04-355-1
5 L
$91.42
Triton X-100
detergent that can be used instead of CHAPS
GE Healthcare
17-1315-01
500 ml
$40.00
2-D Quant Kit
Accurately determine protein concentration
GE Healthcare
80-6483-56
500 assays
$219.00
2-D Clean-Up Kit
Improves quality of 2D results by removing interfering contaminants
GE Healthcare
80-6484-51
50 samples
$219.00
BCA Protein Assay Kit
detergent compatible assay for the colorimetric detection and quantitation of total protein
Pierce
23227
250 assay
$87.00
Urea
protein solubilization; used in rehydration soln., cell lysis , 2X sample buffer and equilibrium buffer
GE Healthcare
17-1314-01
500 gm
$33.00
CHAPS
protein solubilization; used in rehydration soln., cell lysis buffer and 2X sample buffer.
GE Healthcare
17-1314-01
1 GM
$59.00
Thiourea
solubilizes and denatures proteins, using thiourea in addition to urea can further improve solubilization particularly membrane proteins; used in rehydration soln. and 2X sample buffer.
GE Healthcare
RPN6301
100 gm
$52.00
Iodoacetamide
alkylation of thiols preventing reoxidation, which can result in streaking and other artifacts; minimize unwanted reactions of cysteine residues if MALDI will be done later; alkylates residual DTT to revent point streaking and other silver-staining artifacts; used in 2nd equilibrium buffer
GE Healthcare
RPN6302
25 gm
$83.00
Dithiothreitol (DTT)
reductant that cleaves disulphide bonds to allow proteins to unfold completely; used in 2X gel loading buffer, rehydration buffer and 2X sample buffer
GE Healthcare
17-1318-01
1 gm
$33.00

DNPH
(2,4-Dinitro-phenylhydrazine)

a chemical compound that can be used in a chemical test to detect whether an organic compound has a C=O (carbonyl) group.
Sigma
D-199303-25G
25 gm
$21.80
Bromophenol Blue
Tracking dye for 1st and 2nd dimension
GE Healthcare
17-1329-01
10 gm
$35.00

Cy Dye DIGE Fluor
Cy 2

CyDye™ DIGE fluors are dyes for multicolor analysis, offering bright and intense colors with narrow excitation and emission bands. The fluors are spectrally distinct, making them ideal for multicolor detection. They are also available in 25 nM and 5 nM quantities
GE Healthcare
25-8008-60
10 nM/vial
$1526.00

Cy Dye DIGE Fluor
Cy 3

GE Healthcare
25-8008-61
10 nM/vial
$1526.00

Cy Dye DIGE Fluor
Cy 5

GE Healthcare
25-8008-62
10 nM/vial
$1526.00
Immobiline™ DryStrip gels
13 cm,pH 3-5.6 NL
there are 24cm, 18cm, 13cm and 7 cm also available in the same pH ranges.
GE Healthcare
17-6003-55
12 strips
$108.00
Immobiline™
DryStrip 13 cm,
pH 5.3-6.5 NL
GE Healthcare
17-6003-60
12 strips
$108.00
Immobiline™ DryStrip 13 cm,
pH 6.2-7.5 NL
GE Healthcare
17-6003-65
12 strips
$108.00
Immobiline™ DryStrip 13 cm,
pH 7-11 NL
GE Healthcare
17-6003-70
12 strips
$108.00
Immobiline™ DryStrip 13 cm,
pH 3-11 NL
GE Healthcare
17-6003-75
12 strips
$108.00
Immobiline™ DryStrip 13 cm,
pH 4-7 NL
GE Healthcare
17-6001-13
12 strips
$108.00
Immobiline™ DryStrip 13 cm,
pH 6-11 NL
GE Healthcare
17-6001-96
12 strips
$108.00
Immobiline™ DryStrip 13 cm,
pH 3-10 NL
GE Healthcare
17-6001-15
12 strips
$108.00
Immobiline™ DryStrip 13 cm,
pH 3-10
GE Healthcare
17-6001-14
12 strips
$108.00
IPG Buffer, pH 4-7
For use with Immobiline™ DryStrip gels to improve protein solubility. The buffer is specific for whatever pH range you buy. If you use Cy Dyes, you have to use Pharmalyte (see below). Ensures optimal conditions for IEF separation. Eliminates background staining.
GE Healthcare
17-6000-86
1 ml
$47.00
IPG Buffer,
pH 3-10
GE Healthcare
17-6000-87
1 ml
$47.00
IPG Buffer,
pH 3-11 NL
GE Healthcare
17-6004-40
1 ml
$51.00
IPG Buffer,
pH 6-11
GE Healthcare
17-6001-78
1 ml
$47.00
IPG Buffer,
pH 7-11 NL
GE Healthcare
17-6004-39
1 ml
$51.00
Pharmalyte, broad range
pH 2.5-5
For use with Immobiline™ DryStrip gels to improve protein solubility. The buffer is specific for whatever pH range you buy. If you use Cy Dyes, you have to use Pharmalyte. Ensures optimal conditions for IEF separation. Eliminates background staining.
GE Healthcare
17-0451-01
25 ml
$244.00
Pharmalyte, broad range
pH 4-6.5
GE Healthcare
17-0452-01
25 ml
$244.00
Pharmalyte, broad range pH 5-8
GE Healthcare
17-0453-01
25 ml
$244.00
Pharmalyte, broad range
pH 8-10.5
GE Healthcare
17-0455-01
25 ml
$244.00
Pharmalyte, broad range
pH 3-10
GE Healthcare
17-0456-01
25 ml
$244.00
Pharmalyte, Narrow range
pH 4.2-4.9
GE Healthcare
17-0562-01
25 ml
$297.00
Pharmalyte, Narrow range
pH 4.5-5.4
GE Healthcare
17-0563-01
25 ml
$297.00
Pharmalyte, Narrow range
pH 5-6
GE Healthcare
17-0564-01
25 ml
$297.00
Pharmalyte, Narrow range
pH 6.7-7.7
GE Healthcare
17-0566-01
25 ml
$297.00
IPGphore Strip Holder Cleaning Soln. (nonionic)
nonionic detergent for cleaning strip holders and covers
GE Healthcare
80-6452-78
950 ml
$48.00
Immobiline DryStrip Cover Fluid
oil used to cover Immobiline DryStrip and sample for the 1st dimension to prevent drying out strip
GE Healthcare
17-1335-01
1 L
$53.00
DeStreak Rehydration Solution
Improves reproducibility and quallity fo 2-D gels by preventing streaking
GE Healthcare
17-6003-19
5 X 3 ml
$89.00
Bind Silane
Binds the gel to 1 plate. You need all of the gel on a specific plate for spot picking.
GE Healthcare
17-1330-01
25 ml
$92.00
Protogel
for 2nd dimension vertical gels
National Diagnostics
EC-890
1 L
$67.93
Ammonium persulfate
Add to the Protogel for gel polymerization
GE Healthcare
17-1311-01
25 gm
$35.00
TEMED
Add to the Protogel for gel polymerization
GE Healthcare
17-1312-01
25 ml
$29.00
Plastic trays for gels/glass plates

multiple uses; storing 2nd dimension gels and staining 2D gels
VWR
62650-129
6/cs
$49.36
Gel Releasers
Helps pry the 2nd dimension gel sandwich apart
Bio-Rad
165-3320
5/PK
$22.00
SDS
use in equilibration solution and running buffer for 2nd dimension; protein solubilization
Sigma
17-1313-01
100 gm/ea.
$40.00
Glycerol
Stabilizes the pH gradient and stabilizes 2nd D gels
GE Healthcare
17-1325-01
1 L
$49.00
Glycine
use in running buffer for 2nd dimension
GE Healthcare
17-1323-01
500 gm
$35.00
Tris
GE Healthcare
17-1321-01
500 gm
$60.00
ProtoMarkers
Protein marker
National Diagnostics
EC-898
500 ul
$81.00
Deep Purple Protein Stain
Deep Purple™ Total Protein Stain* is an ultra-sensitive, fast, and easy-to-use fluorescent stain for the detection of proteins in gels or blots following electrophoretic separation. It is similar to sypro ruby but gives nicer spots and is a bit cheaper than sypro, though not quite as sensitive. Very similar to SYPRO Ruby
GE Healthcare
RPN6305
5 ml (makes 1 L)
$168.00
Citric Acid
new Deep Purple protocol
Sigma
C0759-500G
500 gm
$22.50
Boric Acid
new Deep Purple protocol
Fisher
A73-500
500 gm
$75.05
SYPRO Ruby
very sensitive fluorescent stain (8-28 ng range) for protein gels that can be detected by UV fluorescence. Very similar to Deep Purple Protein Stain
Molecular Probes (Invitrogen)
S12001
200 ml
$67.00
Krypton Protein Stain
Fluorescent stain, almost as sensitive as Deep Purple and SYPRO Ruby; better background
Fisher
P153071
100 ml
$82.70
ProteoSilver Silver Stain kit
Stable and sensitive silver staining kit
Sigma
PROTSIL1-KT
25 mini gels
$135.50
Coomasie Brilliant Blue R-250
commonly used stain for protein gels; inexpensive and rapid but low sensitivity (100- 1000ng range)
Bio-Rad
161-0400
10 gm
$18.00
SimplyBlue SafeStain
Fast, sensitive and safe Coomassie G250 stain
Invitrogen
LC6060
1 L
$100.00
GelCode Blue Stain Reagent
Coomassie G250 stain without multiple steps for destaining
Pierce
24590
500 ml
$55.00

 


This information is given as a guide to the facilities and instrumentation available in the DNA/Protein Core facility at Georgia State University. If you have any concerns or thoughts about the content of this website please contact: John Houghton (404) 651-0549