Protein Sequencing
The ABI Procise sequencer that has been in use for the past 6 years is being phased out. The Core Facility is moving towards "Protein ID" and sequencing using the ABI 4800 Plus MALDI-TOF/TOF Analyzer along with database analysis (which is significantly more cost-effective). If you need more information, please contact Hyuk-Kyu Seoh or John Houghton to discuss our current protein sequencing capabilities.
ABI Procise Sequencing
Please print out and fill in the form below, and return to Sonja Young.
404-413-5370. At this time, DO NOT leave samples in door slot of NSC 518. Contact Sonja directly for pickup or delivery.
| Protein Sequencing Request Form | |||
|---|---|---|---|
Biotechnology & Drug Design Core Facility, Georgia State University |
Contact: Sonja Young Natural Science Center, 50 Decatur St, Atlanta, GA 30303 |
||
Charges: |
$160 for first 8 cycles; $25 each additional cycle |
||
Contact Name: |
|||
Contact Phone: |
E-mail: |
||
Department/University: |
|||
Address: |
|||
P I's Name: |
Acct / PO # | ||
P I's Signature: |
|||
Sample Name: |
|||
Number of Residue Cycles to run (5 - 20) ___________________ |
|||
Expected Molecular Weight ______________________________ |
|||
Purity % _______________________ by ____________________ |
|||
Likely Contaminants/Modified Residues ____________________ |
|||
________ Protein ________ Fragment __________ Peptide |
|||
________ Hydrophobic _______ Hydrophilic ______Radioactive |
|||
Sample State: Electroblotted ________ PVDF _______ |
Estimated Amount of sample (pmols) _______________________ |
Manufacturer of Membrane _______________________________ |
Last Purification Step _______________________________ |
N-terminal Sequence (if known) _______________________ |
Special Instructions: |
_______________________________ |
_______________________________ |
|
This information is given as a guide to the facilities and instrumentation available in the DNA/Protein Core facility at Georgia State University. If you have any concerns or thoughts about the content of this website please contact: John Houghton (1- 0549: jhoughton@gsu.edu)
