Core Facility (Houghton'04)

Genomics/Affymetrix

Gene Chip Expression
Supply List

Please print out and fill in the form below, and return to Sonja Young
Rm (lab): PSC 661
Cell: (678) 640-9259

Rm (office): PSC 519
Tel: (404) 413-5363

	Following prices apply to academic institutions. Contact Core Personnel for other pricing.
	Charges :
	Full Service :	$400.00/sample + cost of chip (user provides RNA; price includes probe prep, Hybridization, Fluidics and Scan)
	Chip :	price varies with type of chip (contact Sonja for details)
	Probe Prep :	$233.00/sample (includes Probe preparation reagents)
	Hybridization :	$100.00/chip (includes Hybrization reagents)
	Fluidics :	$60.00/Fluidics run (includes Fluidics station wash and stain reagents)
	Scan :	$7.00/chip (Scanner use only)

Affymetrix Request Form

Biotechnology & Drug Design Core Facility, Georgia State University			Contact: Sonja Young Petit Science Center, 161 Jesse Hill Jr. Dr., Atlanta, GA 30303
Contact Name:
Contact Phone:		E-mail:
Department/University:
Address:
Address:
P I's Name:		Acct / PO #
P I's Signature:

Sample Name:
	Type of Microarray chip _________________________________
	Concentration of total RNA ______________________________

Supplies

Supply cost is to be used only as a guide. It is a website price (as of 1/3/07) and might not include any University discounts, shipping and handling.

Item	Description	Company	Catalog #	Unit	Cost/Unit
Human Genome Array	U133 Set (5 HG-U133A & 5 HG-U133B)	Affymetrix	900370	10 chips	$4000.00
Human Genome Array	Human Genome U133 A	Affymetrix	900366	5 chips	$2000.00
Human Genome Array	Human Genome U133 B		900366	5 chips	$2000.00
B. subtilis Array		Affymetrix	900813	5 chips	$1800.00
Yeast Genome array	Yeast S98	Affymetrix	900256	5 chips	$1500.00
P. aeruginosa array		Affymetrix	900339	5 chips	$1500.00
E. coli Antisense array		Affymetrix	900381	5 chips	$1500.00
Enzo Bioarray High Yield RNA Transcript Labeling kit (T7)	Target prep and labeling Contains: vial 1: 10 X HY Reaction Buffer vial 2: 10X Biotin-Labeled Ribonucleotides vial 3: 10X DTT vial 4: 10X RNase Mix vial 5: 20X T7 RNA Polymerase		42655-20A	20 reactions	$1085.00
T7-Oligo (dT) Promoter Primer Kit	Target Prep and Labeling; included in 1 and 2 cycle kits	Affymetrix	900375	150 reactions	$975.00
IVT Labeling Kit	Target Prep and Labeling	Affymetrix	900449	30 reactions	$1500.00
One-cycle cDNA Synthesis Kit	Target Prep and Labeling	Affymetrix	900431	30 reactions	$1710.00
Two-cycle cDNA Synthesis kit	Target Prep and Labeling	Affymetrix	900432	30 reactions	$2460.00
Eukaryotic Poly-A RNA Control Kit	Target Prep and Labeling	Affymetrix	900433	100 reactions	$300.00
Superscript II RNase H Reverse Transcriptase	Target Prep and Labeling	Invitrogen	18064-014	50 reactions	$219.00
E.coli DNA Polymerase I	Target Prep and Labeling	Invitrogen	18010-025	25 reactions	$205.00
E. coli DNA ligase	Target Prep and Labeling	Invitrogen	18052-019	10 reactions	$33.00
E. coli RNase H	Target Prep and Labeling	Invitrogen	18021-071	60 reactions	$334.00
T4 DNA Polymerase	Target Prep and Labeling	Invitrogen	18005-025	25 reactions	$196.00
5X Second strand buffer	Target Prep and Labeling	Invitrogen	10812-014		$76.00
10 mM dNTP	Target Prep and Labeling	Invitrogen	18427-013	33 reactions	$64.00
Herring Sperm DNA	Hybridization	Promega	D1811	333 reactions	$41.00
GeneChip Eukaryotic Hybridization Control kit	Hybridization components: 20X Hybridization Controls 3 nM Control Oligo B2	Affymetrix	900454	30 reactions	$300.00
BSA	Staining	Invitrogen	15561-020		$153.00
R-Phycoerythrin Streptavidin	Staining	Molecular Probes	S-866	83 reactions	$225.00
Goat IgG, Reagent Grade	Staining	Sigma-Aldrich	I5256	166 reactions	$116.00
Anti-Streptavdin antibody (goat) biotinylated	Staining	Vector Laboratories	BA-0500		$90.00
PBS, pH 7.2	Staining	Invitrogen	20012-027	100 reactions	$13.25
20 X SSPE	Staining	BioWittaker	16-010Y	3.3 expt.	$38.00
10 % surfact-Amps20 (Tween 20)	Staining	Pierce	28320	60 reactions	$88.00

	This information is given as a guide to the facilities and instrumentation available in the DNA/Protein Core facility at Georgia State University. If you have any concerns or thoughts about the content of this website please contact: John Houghton (404) 413-5390

This information is given as a guide to the facilities and instrumentation available in the DNA/Protein Core facility at Georgia State University. If you have any concerns or thoughts about the content of this website please contact: John Houghton (404) 413-5390