Protein Sequencing Contact: Sonja YoungRm: NSC 518 Tel: (404) 413-5379 Protein sequencing request form |
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Charges: |
$160 for first 8 cycles; $25 for each additional cycle when rotor speed is above 3000 rpm |
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The ABI Procise protein sequencer sequentially cleaves amino acids from the N-terminus of a protein or peptide. The system then separates and identifies the cleaved amino acids and analyzes the data. The ABI Procise sequencer that has been in use for the past 6 years is being phased out. The Core Facility is moving towards "Protein ID" and sequencing using the ABI 4800 Plus MALDI-TOF/TOF Analyzer along with database analysis (which is significantly more cost-effective). If you need more information, please contact Hyuk-Kyu Seoh or John Houghton to discuss our current protein sequencing capabilities. Websites: Procise Protein Sequencing System Edman Sequencing Sample Prep: Run an SDS PAGE gel with 1ug protein per lane (purity 90% or above). Blot bands onto PVDF membrane with Caps buffer (CAPS SigmaUltra C6070-100G). For the best results, provide 2 to 4 samples/lane from the same protein band that you are interested in. This information is given as a guide to the facilities and instrumentation available in the DNA/Protein Core facility at Georgia State University. If you have any concerns or thoughts about the content of this website please contact: John Houghton (404) 413-5390
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