Genomics/Affymetrix Gene Chip Expression Contact: Sonja YoungRm: NSC 518 Tel: (404) 413-5379 Affymetrix Request Form |
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Charges : |
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Full Service :
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$400.00/sample + cost of chip (user provides RNA; price includes probe prep, Hybridization, Fluidics and Scan) |
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Chip : |
price varies with type of chip (contact Sonja for details) |
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Probe Prep : |
$233.00/sample (includes Probe preparation reagents) |
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Hybridization : |
$100.00/chip (includes Hybrization reagents) |
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Fluidics : |
$60.00/Fluidics run (includes Fluidics station wash and stain reagents) |
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Scan : |
$7.00/chip (Scanner use only) |
GeneChip® arrays enable scientists to attain ambitious goals from identifying genetic variations associated with disease to discovering new drug targets. Website: Affymetrix Steps for an Affymetrix Experiment Sample Prep Supplies Steps for an Affymetrix Experiment Eukaryotic/Prokaryotic samples EUKARYOTIC SAMPLE AND ARRAY PROCESSING 1. Target Preparation 2. Preparation of controls for Arrays 3. Target Hybridization 4. Washing, Staining and Scanning 5. General Analysis PROKARYOTIC SAMPLE AND ARRAY PROCESSING 1. Direct Labeling of enriched E. coli mRNA 2. Preparation of Control Spike Transcripts for E. coli Genome Array 3. E. coli Target Hybridization 4. Washing, Staining and Scanning 5. General Analysis Sample Prep: Starting material (Eukaryotic): High Quality total RNA (5.0µg – 40.0µg) cDNA synthesis protocol: Affymetrix recommends starting with a minimum of 5 µg of total RNA at a minimum concentration of 0.5 µg/µL. Determining RNA Concentration: After purification the RNA concentration is determined by absorbance at 260nm on a spectrophotometer (one absorbance unit = 40 µg/mL RNA) The A260/A280 ratio should be approximately 2.0, with ranges between 1.8 to 2.1 considered acceptable. This information is given as a guide to the facilities and instrumentation available in the DNA/Protein Core facility at Georgia State University. If you have any concerns or thoughts about the content of this website please contact: John Houghton (404) 413-5390
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